The dynamic complex of cytochrome c6 and cytochrome f studied with paramagnetic NMR spectroscopy

The dynamic complex of cytochrome c6 and cytochrome f studied with paramagnetic NMR spectroscopy.

The rapid transfer of electrons in the photosynthetic redox chain is achieved by the formation of short-lived complexes of cytochrome b6f with the electron transfer proteins plastocyanin and cytochrome c6. A balance must exist between fast intermolecular electron transfer and rapid dissociation, which requires the formation of a complex that has limited specificity. The interaction of the solub...

Properties of the copper associated with cytochrome oxidase as studied by paramagnetic resonance spectroscopy.

There appears to be general agreement that cytochrome oxidase preparations obtained by various procedures contain amounts of copper in the range of the heme content of this enzyme (l-9). Various workers disagree, however, on the significance of this finding (6-10). Whereas several investigators hold the view that the copper associated with cytochrome oxidase has properties that would qualify it...

Role of hydrophobic interactions in the encounter complex formation of the plastocyanin and cytochrome f complex revealed by paramagnetic NMR spectroscopy.

Protein complex formation is thought to be at least a two-step process, in which the active complex is preceded by the formation of an encounter complex. The interactions in the encounter complex are usually dominated by electrostatic forces, whereas the active complex is also stabilized by noncovalent short-range forces. Here, the complex of cytochrome f and plastocyanin, electron-transfer pro...

Chapter IV Paramagnetic NMR using spin-labeled proteins to study the structure of the complex between cytochrome f and plastocyanin

Solution structure and dynamics of the complex between cytochrome c and cytochrome c peroxidase determined by paramagnetic NMR.

The physiological complex of yeast cytochrome c peroxidase and iso-1-cytochrome c is a paradigm for biological electron transfer. Using paramagnetic NMR spectroscopy, we have determined the conformation of the protein complex in solution, which is shown to be very similar to that observed in the crystal structure [Pelletier H, Kraut J (1992) Science 258:1748-1755]. Our results support the view ...